xeno light dir dye  (Revvity)

Journal: Pharmaceutics

Article Title: Design and Evaluation of Autophagy-Inducing Particles for the Treatment of Abnormal Lipid Accumulation

doi: 10.3390/pharmaceutics14071379

Figure Lengend Snippet: In vivo and organ DiR fluorescence imaging of genetically obese ob/ob mice following a single IV injection (120 µL) of soluble fluorophore or fluorescent particles (0.25% w/v ). ( a ) Autophagy-inducing fluorescent particles (NP DiR T-B) and plain fluorescent particles (NP DiR) accumulate in the liver over the course of 72 h. Images are representative of scans from 3–4 mice per group. ( b ) Quantification of whole-body fluorescence up to 72 h post a single IV injection. The percentage values reported are calculated from the 5 min timepoint, when the maximal fluorescence was observed, and are reported as mean ± s.e.m. Results were analyzed using one-way ANOVA followed by Sidak’s multiple comparison test, which revealed no significant difference between the NP DiR and the NP DiR T-B group for all timepoints. ( c , d ) Organ biodistribution of fluorescent NPs 24 h after a single intravenous (IV) injection in ob/ob mice. n = 3–4 mice per group. Ex vivo DiR fluorescence imaging on main organs (liver, spleen, pancreas and kidneys) ( c ) and quantification of DiR fluorescence ( d ). The percentage values reported are calculated from total organ fluorescence and presented as mean ± s.e.m ( d ).

Article Snippet: Mice were immediately injected in the retro-orbital vein with 120 µL of either free fluorophore (Xenolight DiR-Fluorescent Dye, PerkinElmer, Waltham, MA, USA, 125964), fluorescent nanoparticles (NP DiR, Near Infra-Red Fluorescent i-Particles ® , Adjuvatis, France) or fluorescent Tat-Beclin nanoparticles (NP DiR T-B) of 0.25% w/v solid content.

Techniques: In Vivo, Fluorescence, Imaging, IV Injection, Comparison, Ex Vivo

Journal: PLoS ONE

Article Title: IFNγ-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes

doi: 10.1371/journal.pone.0255778

Figure Lengend Snippet: (A) Representative low-power images of Xenolight DiR-labelled IFNγ-DC-EVs (DiR-EVs) fluorescence in the rostral portion of the nose/brain (Left-to-right: sham, < one hour post nasal administration of DiR-EVs, one-to-two hours post nasal administration, > two hours post nasal administration). Sham controls consisted of exosome-free (non-conditioned) media prepared in the same manner as conditioned media for labelling of EVs. (B) Quantification of radiant efficiency shows significantly (* p = 0.040, <0.001 and 0.025, respectively) higher fluorescence in the forebrain area of animals nasally administered DiR-EVs versus sham. (C) Imaging of organs (Top row: lung, kidney, liver; bottom row: spleen and brain) was performed for whole brain (therapeutic target) and liver, kidney, spleen (clearance/toxicity) and lung (for error in nasal delivery) at one hour post nasal administration. Representative images of Sham (Left) and DiR-EV (Right) organs are shown. (D) Quantification of radiant efficiency showed significantly (* p = 0.018) increased signal in nasal administration DiR-EV whole brain, but not in other organs. To better visualize DiR-EV uptake, explanted brains were serially dissected into three mm sections. (E) Representative images of Sham (Left) and DiR-EV (Right) after nasal administration are shown, with coronal brain sections (1–8), a sagittal section to the lower left and two regions of upper (left) and mid (right) cervical spinal cord to the lower right. (F) Quantification of radiant efficiency showed significantly (* p = 0.003–0.048) increased signal in all brain sections except olfactory bulb ( n = 5-7/group; C-F).

Article Snippet: EVs were labelled with Xenolight DiR (#125964; PerkinElmer), a lipophilic near infrared dye that stably stains cytoplasmic membranes with negligible dye transfer between cells.

Techniques: Fluorescence, Imaging

Journal: PLoS ONE

Article Title: IFNγ-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes

doi: 10.1371/journal.pone.0255778

Figure Lengend Snippet: (A) Representative images of explanted organs at one hour post-administration of Xenolight DiR-labelled EVs. (Top row: liver and lung; middle: kidney; bottom row: spleen and brain) [Left to right: Sham, nasally administered (NA) DiR-EVs, intravenously delivered (IV) DiR-EVs]. Sham controls consisted of intravenously administered Xenolight DiR-labelled exosome-free (non-conditioned) medium prepared in the same manner as conditioned media for labelling of EVs. (B) Quantification shows significantly higher (* p <0.001) signal in nasally administered and intravenously delivered DiR-EV brains, and significantly higher (* p = 0.027) signal in intravenously delivered DiR-EV liver. There was also a significant difference (# p = 0.050) between liver DiR-EV fluorescence in intravenously versus nasally treated animals. No other organ measured showed significant differences. (C) Representative images of 3 mm thick sagittal sections of brain [Left to right: sham, nasally administered DiR-EVs, intravenously delivered DiR-EVs]. (D) Quantification of sagittal sections showed significantly (* p < 0.001) increased signal in both nasally administered and intravenously delivered DiR-EV administered animals versus sham. (E) Quantification of additional brain areas showed no significant difference in CNS distribution between delivery routes (Sections 1–7 defined as in ). ( n = 3-4/group; A-E).

Article Snippet: EVs were labelled with Xenolight DiR (#125964; PerkinElmer), a lipophilic near infrared dye that stably stains cytoplasmic membranes with negligible dye transfer between cells.

Techniques: Fluorescence